A real-time polymerase chain reaction (real-time PCR, or qPCR when used quantitatively) is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR).

Aug 14, 2025 · Real-Time PCR is a technique used to monitor the progress of a PCR reaction in real-time. At the same time, a relatively small amount of PCR product (DNA, cDNA or RNA) can be …

This section provides an overview of real-time PCR, reverse-transcription quantitative PCR techniques, and the choice of instruments that Bio-Rad offers for these techniques.

Real-time PCR is a modification of the conventional PCR technique, enabling real-time monitoring of the PCR progress. The real-time PCR systems are dependent on a fluorescent reporter of the probe …

Real-time PCR combines PCR amplification and detection into a single step. This eliminates the need to detect products using gel electrophoresis, and more importantly it enables the method to be truly …

Dec 26, 2019 · Real-time PCR also called quantitative PCR (qPCR), is a variant of standard polymerase chain reaction in which amplification and simultaneous quantitation of a target DNA is done in the …

Sep 21, 2025 · Real-time PCR, also known as quantitative PCR (qPCR), is a molecular biology technique that allows for the amplification, detection, and quantification of DNA or RNA in real-time …