Subtilisin can cause "enzymatic detergent asthma". People who are sensitive to Subtilisin (Alcalase) usually are also allergic to the bacterium Bacillus subtilis.

Nov 26, 2025 · Subtilisin remains a key enzyme in enzymology due to its adaptability and broad applications. This review synthesizes recent progress from 2020 to 2025, integrating bibliometric …

Biochemical scrutiny unveiled the enzyme's response, with an optimal pH at 9 and temperature peak at 60 ℃. Various surfactants, metal ions, organic solvents and inhibitors exhibited notable efficacy.

Mar 4, 2021 · Subtilisin is a serine endopeptidase, type example of peptidase family S8. It contains no cysteine residues (although these are found in homologous enzymes).

Subtilisin refers to a type of extracellular serine endopeptidase enzyme that was first isolated from the bacterial species Bacillus subtilis. It is part of a large family of homologous proteins known as …

Mar 4, 2021 · The enzymes of the present invention are generally produced by recombinant methods, in particular by expression of a subtilisin BPN' DNA which has been mutated such that upon expression …

Mar 21, 2025 · This article undertakes an exhaustive examination of wild subtilisin enzyme, covering purification, biochemical delineation, analytical techniques, and practical implementations.