Restriction enzyme fingerprinting was applied to 72 transferable trimethoprim resistance plasmids to examine aspects of their epidemiology and molecular relatedness. These plasmids had previously been ...

The DNA is preferentially purified from other cellular components such as genomic DNA and RNA. Typical DNA recoveries range between 0.6 and 1 µg from 3.0 mL of bacterial culture. The purified DNA is ...

Research by Professor of Chemical and Biomolecular Engineering Huimin Zhao and graduate student Behnam Enghiad at the University of Illinois is pioneering a new method of genetic engineering for basic ...

Key to Invitrogen's Gateway technology is the "entry clone," which contains a fragment of interest in a recombinase-ready plasmid. Researchers can quickly and efficiently move between expression ...

The Gibson Assembly® method has quickly become an invaluable tool for molecular cloning workflows due to its flexibility and efficiency. It allows for the cloning of multiple fragments directly into ...

Molecular cloning is a technique used to create DNA experimental tools that are expressed in either bacterial or mammalian cells. Molecular cloning is defined as the isolation and amplification of a ...

(Nanowerk News) Plasmids have extensive use in basic and applied biology. These small, circular DNA molecules are used by scientists to introduce new genes into a target organism. Well known for their ...