With clean samples and moderate-to-high target levels, qPCR and digital PCR give comparable results. Digital PCR’s advantages show up with low-abundance targets, variable inhibitors, small fold ...

In this Innovation Spotlight, Marwan A. Alsarraj, global segment manager at Bio-Rad Laboratories, discusses how digital PCR eliminates the need for standard curves while achieving high sensitivity and ...

According to a recent report on the gene-amplification technologies market from Global Industry Analysts, there are close to 70,000 bioresearchers using real-time quantitative PCR (qPCR) in North ...

Researchers from Critical Analytics for Manufacturing Personalized-Medicine (CAMP), an Interdisciplinary Research Group (IRG) at the Singapore-MIT Alliance for Research and Technology (SMART), MIT's ...

In a recent medRxiv* preprint manuscript, researchers from Singapore and the United States report the development of a digital CRISPR method for the sensitive, specific, and rapid detection of viral ...

Quantitative polymerase chain reaction (qPCR) remains one of the most widely used techniques for nucleic acid quantification, but generating accurate, reproducible results depends on careful assay ...

A single molecular biology bench might hold three PCR instruments that look broadly similar: a standard thermal cycler, a real-time qPCR system, and a digital PCR platform. They all amplify DNA. They ...

Quantitative polymerase chain reaction (qPCR) has become a cornerstone technique for the sensitive and precise measurement of gene expression. By monitoring DNA amplification in real time, qPCR ...

Relative and absolute quantification are two approaches used mainly in molecular biology (especially qPCR, gene expression analysis, and mass spectrometry-based proteomics) to measure the amount of a ...

Definition: DNA amplification is the laboratory process of making many copies of a selected DNA sequence from a very small starting amount. It allows DNA that would otherwise be too scarce to detect ...